Bioengineering of Animal/Human Cells
Biotechnologie mit tierischen und menschlichen Zellen
8 LPSoSeEN
Voraussetzungen
Zwingend, im Wortlaut
Only applies to the Master Biotechnology PO 2018 with 4 semesters standard period of study: Successfull completion of module • 12739 - Research Internship
Empfohlen, im Wortlaut
Basic knowledge in Cell biology and Biochemistry. Participation in the laboratory course 12771 - Cells and Tissues - Culture and Evaluation is highly recommended.
Inhalte
Culture of cells in Monolayer (2D) of different cell types (including differentiation). - culture and differentiation of macrophages and monocytes - detection and visualization of inflammasome activation - microscopy of blood smears and immune organs - culture and differentiation of myogenic cells - functional analysis of specific genes in myogenic cells Contents of the seminar: Deepening the knowledge of the theoretical background used in the Labcourse.
Lernziele
Students expand their professional competencies in training the skills in sterile cell culture techniques and practice the performance of special cell assays. Furthermore they broaden their methodological background in analysis, interpretation, and reporting of lab data. By working in small groups the students train also soft skills like the ability to work and communicate in a team. The special task to sum up and discuss the experimental results train additional soft skills like the ability to communicate orally and written to individuals and groups and to tailor the communication to the level and experience of the audience. The students use appropriate software, graphics, and other tools to clarify complex information. Moreover, the accompanying seminar will train methodological competencies (e.g. media skills), and soft skills like presenting special facts and data and to communicate in a group.
Prüfungsleistung
weeks 1-3 (Prof. Manuela Rossol): • Lab work: 2 experiments, performance, time managment, critical evaluation of data (12.5%) • Presentation and discussion in the seminar, duration depends on the topic (12.5%) • Protocol: Introduction, Methods, Results and Discussion, min, 10 pages (12.5%) • written exam, duration: 45 min (12.5%) weeks 4-6 (Prof. Julia von Maltzahn): • Lab work: 2 experiments, performance, time managment, critical evaluation of data (12.5%) • Presentation and discussion in the seminar, duration depends on the topic (12.5%) • Protocol: Introduction, Methods, Results and Discussion, min. 10 pages (12.5%) • written exam, duration: 45 min (12.5%) The duration of the presentation will be announced at the beginning of the course.
Literatur (5)
- Freshney, Ian: Culture of Animal Cells: A Manual of Basic Technique and Specialized A pplications ; 2010
- Lindl, Toni.; Gstraunthaler G.: Zell- und Gewebekultur. Von den Grundlagen zur Laborbank; Spektrum Akademischer Verlag, 6. Auflage, 2008
- Schmitz, Sabine: Der Experimentator Zellkultur, Spektrum Akademischer Verlag 2011
- Specific original scientific publications
- Manuals (SOPs) for cell culture & cell analytics of the Labs of Prof. U. Anderer, Prof. Manuela Rossol and Prof. Julia von Maltzahn
Bemerkungen
The module takes place as a 6 week block course (split in 2 parts of 3 weeks duration each).
Quelle: Modulbeschreibung der BTU · abgerufen 19.10.2026Original