Module

Voraussetzungen

Inhalte

Microbial Metabolism and Regulation As a general principle all statements presented in the lectures are based on conclusions drawn from original data. Topics: a) Mutations affecting metabolic pathways: (conditional) auxotrophy; supplementation; glycine biosynthesis; definded growth media; b) Isoenzymes: isocitrate DHs; serine hydroxymethyltransferases; carbon flux analysis; subcellular compartmentation; aspartate kinases of E. coli c) Regulation of metabolism: lac, ara, trp operon; repression, induction, attenuation, mRNA half-life control;; adenylation of glutamine synthetase; phosphorylation; feed back inhibition d) Circadian clocks: control of photosynthesis and nitrogenase in Synechococcus and Anabaena; sporulation in Neurospora e) Reporter systems: lacZ; green fluorescent protein; organelle targeting; f) Quorum sensing: biofilms; signal systems in Gram-positive and Gram-negative bacteria;

Lernziele

Goal of the lecture is • repetition of basics in bacterial and fungal metabolism and its regulation • in depth understanding of selected topics based on recent original papers and current reviews • to understand how conclusions are drawn from data, especially experimental data • to understand how independent research strategies can lead to overlapping evidences • to understand the difference qualitative and quantitative experimental approaches • to understand the route starting from a scientific question over experimental data to a model explaining complex phenomena of microbiology • to become able to work as a PhD student

Prüfungsleistung

Written Examination (120 min)

Bemerkungen

"Recommended Placement in the Study Plan" Biotechnology (Master of Science, 3 Semester Version): Master 1st year course Biotechnology (Master of Science, 4 Semester Version): Master 2nd year course