Methods in Synthetic Microbiology
Methoden der synthetischen Mikrobiologie
8 CPSummerEN
Prerequisites
Mandatory, as written
Only applies to the Master Biotechnology PO 2018 with 4 semesters standard period of study: Successfull completion of module • 12739 - Research Internship
Recommended, as written
Basic lab experience; basis knowledge in molecular biology, biochemistry, microbiology.
Contents
• cultivation of Microorganisms under suitable conditions • isolation and modification of DNA • constructing genetic tools or overexpression vectors • create relevant synthetic microorganisms such as genetically modified strains for biotechnological purposes or further investigations
Learning outcomes
After completing the module, the students will be able to present the current literature on a research area in a summarized form. By presenting and discussing their results from the laboratory course they are able to critically question research results. In the practical course, students have to work on sophisticated projects which need competences such as teamwork, organized project planning (project management) and analytical abilities. This course aims to teach diverse techniques and methods used for genetic engineering, molecular cloning, expression in procaryotic or eucaryotic systems, conceptional thinking, independent preparation, execution, evaluation of experiments, and adequate documentation.
Assessment
After completing the experiments, each student submits a manuscript about the experimental results and give an oral presentation and discussion. • lab performance: 20% • manuscript (max. 10 pages): 40 % • oral presentation and discussion (15 min): 40 %
Literature (1)
- 1. Campbell Biologie (ISBN-10: 3868943668) 2. Brock Biology of Microorganisms (ISBN-10: 1292404795) 3. Der Experimentator Molekularbiologie / Genomics (ISBN-10: 3642346359) 4. Molecular Cloning: A Laboratory Manual (ISBN-10: 1936113422)
Remarks
The lab performance (theoretical preparation, organization and execution in the lab, time management) of each student will be observed. For safety reasons we will evaluate the theoretical preparation of each student occasionally. We therefore reserve the right to interrupt laboratory work and experiments can be continued when successful background knowledge is confirmed.
Source: BTU's module description · retrieved 28 Sep 2026Original